High prevalence of mixed infections by Helicobacter pylori in Hong Kong: metronidazole sensitivity and overall genotype.
نویسندگان
چکیده
BACKGROUND Diversity in metronidazole susceptibility and genotypes of Helicobacter pylori have been reported with varying results in different areas. AIMS To investigate the prevalence of multiple strain infection in a symptomatic Chinese population and to determine the metronidazole susceptibility pattern and genotypic characteristics of these infecting strains. METHODS Gastric biopsies from antrum, body and cardia were taken during upper endoscopy in symptomatic patients referred to our department. Pooled cultures and single colony isolates were obtained and tested for metronidazole susceptibility and random amplified polymorphic DNA (RAPD) fingerprint patterns. RESULTS A total of 461 isolates were successfully cultured from 46 patients. Fifty-seven per cent of subjects had metronidazole-resistant strains. Among them, 77% carried a mixture of sensitive and resistant strains, non-uniformly distributed in the gastric mucosa. Mixed genotypes were found by RAPD typing in 24% of subjects. These did not correlate with the metronidazole susceptibility/resistance pattern. CONCLUSION H. pylori infections with mixed metronidazole sensitive/resistant strains and mixed genotypes are common in Hong Kong. This makes it prudent to use bacterial strains from several biopsy sites when testing for traits such as drug resistance or virulence in relation to disease.
منابع مشابه
Title High prevalence of mixed infections by Helicobacter pylori in Hong Kong: Metronidazole sensitivity and overall genotype
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High prevalence of Helicobacter pylori infection with dual resistance to metronidazole and clarithromycin in Hong Kong.
BACKGROUND Metronidazole resistance is a common problem in most Asian countries, and clarithromycin has been widely used in Hong Kong. AIM To determine the prevalence of Helicobacter pylori strains resistant to metronidazole and clarithromycin in Hong Kong and to assess the effect on eradication rates. Also to determine the genetic mutation in relation to phenotypic divergence in clarithromyc...
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BACKGROUND A study was conducted to evaluate the prevalence of metronidazole resistant Helicobacter pylori strains among the Chinese in Hong Kong. The efficacy of the triple therapy that contains metronidazole as one of the anti-microbial agents in eradication of the metronidazole susceptible and the metronidazole resistant strains was also assessed. METHODS Culture for H pylori was attempted...
متن کاملTitle High prevalence of Helicobacter pylori infection with dual resistance to metronidazole and clarithromycin in Hong Kong
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Investigation of frxA gene mutations in metronidazole resistant Helicobacter pylori strains in Tabriz City
Background: Due to the high rate of Helicobacter pylori infection in our country and its treatment failure, and also because of the high prevalence of antibiotic resistance of this bacterium, evaluating causes of Helicobacter pylori resistance to metronidazole and its effect on the therapeutic course is necessary. Materials and methods: A biopsy sample was obtained from 275 suspected patients r...
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عنوان ژورنال:
- Alimentary pharmacology & therapeutics
دوره 15 4 شماره
صفحات -
تاریخ انتشار 2001